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KMID : 0644120050180010181
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Journal of Oriental Gynecology
2005 Volume.18 No. 1 p.181 ~ p.191
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The effect of -sitosterol proliferation and apoptosis in human uterine leiomyoma cells
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Park Young-Sun
Baek Seung-Hee
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Abstract
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Purpose :β-sitosterol is kind of phytosterols or plant which are structurally similar to cholesterol. This study was aimed to investigate the inhibitory effect of the β-sitosterol on the proliferation of human uterine leiomyoma cells and the expression of gene related the mechanism of cell apoptosis.
Methods :We counted the number of death cells treated with indicated time of the β-sitosterol and investigated cell death rate by cell count assay. Furthermore, flow cytometry analysis and DNA fragmentation assay were used to dissect between necrosis and apoptosis. and then we observed the differential gene expression by western blot analysis.
Results :1)The inhibitory effect on the growth of uterine leiomyoma cell treated with the β-sitosterol 16 μM was increased in a time dependent. 2)The result of flow cytometry analysis, subG1 phase arrest related cell apoptosis was investigated 16.97% in uterine leiomyoma cell treated with the β-sitosterol 16 μM and showed the fashion of proportional time dependent. 3)The gene expression of p27, p21 related cell cycle was increased according to increasing time interval but cyclin E-CDK2 complex was decreased expression. 4)The character of apoptosis, DNA fragmentation was significantly observed on the time dependent. 5)The expression of pro-caspase 3 and PARP were decreased dependent on treatment with time dependent.
Conclusion :This study showed that the β-sitosterol have the inhibitory effect on the proliferation of human uterine leiomyoma cell and the effect was related with apoptosis.
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KEYWORD
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-sitosterol, uterine leiomyoma, spatholobus suberectus Dunn
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